This marks the second selection for both student-athletes and the third straight year the Lions have posted multiple players on an all-region squad.
Senior forward Katie Ruesterholz picked up first team honors, while junior defender Maeve Doherty was selected to the second team and first-year midfielder Louisa Hollander was named honorable mention, respectively.
The Lions wrap up the 2016 season against the Crimson, who wrapped up the Ivy League title last weekend.
The contest will be held on Saturday, November 19 at 2:30 p.m. at Old Dominion University.
Katie Ruesterholz became Columbia's all-time leader in goals with a pair of scores, while the team tied a program record for most tallies in a single-game.
The Bulldogs took the lead in the first half and played stingy defense in the second, holding the Lions to just four second half shots to pick up the win.
Saturday showdown with Yale set for 12 p.m. before Senior Day takes place Sunday against LIU Brooklyn.
Ruesterholz picked up the honor after becoming the Lions' all-time scoring leader in a win over Dartmouth Friday night.
Columbia put on an offensive display, recording a season-high 31 shots and scoring four second half goals to top Dartmouth, 5-1.
The Lions return to the Baker Athletics Complex for a Friday night showdown with the Big Green.
Columbia Athletics invites all alumni, friends and family members to campus this weekend as Columbia University will host its annual 2016 Homecoming weekend.
Bucknell scored four unanswered second half goals to erase a two-goal deficit and claim a 4-3 victory over Columbia.